Device
Part:BBa_K2936009:Design
Designed by: Jiang Weiyan Group: iGEM19_ZJUT-China (2019-10-09)
light-controlled repressible system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 605
Illegal NgoMIV site found at 677
Illegal NgoMIV site found at 767
Illegal NgoMIV site found at 785
Illegal NgoMIV site found at 1297
Illegal NgoMIV site found at 1590
Illegal NgoMIV site found at 1684
Illegal AgeI site found at 319
Illegal AgeI site found at 1465 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1354
Illegal BsaI.rc site found at 218
Design Notes
1. Is there a simple method to detect the expression of repressor protein?Thus the GFP gene is retained to detect the expression level of lacI protein. 2. When linking gene fragments, homologous arms need to be designed on primers.
Source
The light-controlled genes such as J23100 promoter, yf1 protein and FixJ protein come from the plasmid dusk-egfp-puc57 stored in E. coli from 2018-zjut, while the repression system of lactose operon come from pET28b.